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Bioregenerative life support systems (BLSS) may be necessary for long-term space missions due to the high costs of lifting supplies and equipment into orbit. Much of the recycling to be done in a BLSS involves microbial activity. Although most studies to date have used a culture-based approach to characterize bacteria in BLSS under development, recently work has begun utilizing non-culture-based, DNA approaches to elucidate which microbes are present. In this study, we investigated whether replicate reactors develop replicate microbial communities using a 16S rRNA gene approach and terminal restriction length polymorphism analysis for tubular, nitrifier reactors in use at JSC. Our result suggests that both individual reactor and temporal signals can be detected in the microbial populations. This information may lead to optimization of inoculation procedures and reactor operations conditions to increase predictability and reliability of biological systems.

Biological water processors are currently being developed for application in microgravity environments. Work has been performed to develop a single-phase, gravity independent anoxic denitrification reactor for organic carbon removal [1]. As a follow on to this work it was necessary to develop a gravity independent nitrification reactor in order to provide sufficient nitrite and nitrate to the organic carbon oxidation reactor for the complete removal of organic carbon. One approach for providing the significant amounts of dissolved oxygen required for nitrification is to require the biological reactor design to process two-phase gas and liquid in micro-gravity. This paper addresses the design and test results overview for development of a tubular, two-phase, gravity independent nitrification biological water processor.

The Equivalent System Mass (ESM) metric developed by NASA describes and compares individual system impact on a closed system in terms of a single parameter, mass. The food system of a Mars mission may encompass a large percentage of total mission ESM, and decreasing this ESM would be beneficial. Yeast breads were made using three methods (hand & oven, bread machine, mixer with dough hook attachment & oven). Flat breads were made using four methods (hand & oven, hand & griddle, mixer with dough hook attachment & oven, mixer with dough hook attachment & griddle). Two formulations were used for each bread system (scratch ingredients, commercial mix). ESM was calculated for each of these scenarios. The objective of this study was to compare the ESM of yeast and flat bread production for a Martian surface outpost. Method (equipment) for both types of bread production was demonstrated to be the most significant influence of ESM when one equipment use was assumed.

Engineers at the Johnson Space Center (JSC) are using innovative strategies to design the TCS for the Bio-regenerative Planetary Life Support Systems Test Complex (BIO-Plex), a regenerative advanced life support system ground test bed. This paper provides a current description of the BIO-Plex TCS design, testing objectives, analyses, descriptions of the TCS test articles expected to be tested in the BIO-Plex, and forward work regarding TCS. The TCS has been divided into some subsystems identified as permanent “infrastructure” for the BIO-Plex and others that are “test articles” that may change from one test to the next. The infrastructure subsystems are the Heating, Ventilation and Air-Conditioning (HVAC), the Crew Chambers Internal Thermal Control Subsystem (CC ITCS), the Biomass Production Chamber Internal Thermal Control Subsystem (BPC ITCS), the Waste Heat Distribution Subsystem (WHDS) and the External Thermal Control Subsystem (ETCS).

The development of an optimum regenerative Advanced Life Support (ALS) system for future Mars missions has been a crucial issue in the space industry. Considering the numerous potential technologies for subsystems with the complexity of the Air Revitalization System (ARS), Water Reclamation System (WRS), and Waste Management System of the Environmental Control and Life Support System (ECLSS), it will be time-consuming and costly to determine the best combination of these technologies without a powerful sizing analysis tool. Johnson Space Center (JSC), therefore, initiated the development of ALSSAT using Microsoft® Excel for this purpose. ALSSAT has been developed based upon the ALS Requirement and Design Definition Document (Ref. 18). In 1999, a paper describing the development of ALSSAT with its built-in ARS mass balance model (Ref. 21) was published in ICES.

The work presented in this paper summarizes the early results of an integrated advanced water recovery system test conducted by the Crew and Thermal Systems Division (CTSD) at NASA-Johnson Space Center (JSC). The system design and the results of the first two months of operation are presented. The overall objective of this test is to demonstrate the capability of an integrated advanced water recovery system to produce potable quality water for at least six months. Each subsystem is designed for operation in microgravity. The primary treatment system consists of a biological system for organic carbon and ammonia removal. Dissolved solids are removed by reverse osmosis and air evaporation systems. Finally, ion exchange technology in combination with photolysis or photocatalysis is used for polishing of the effluent water stream. The wastewater stream consists of urine and urine flush water, hygiene wastewater and a simulated humidity condensate.

In preparation for future planetary exploration, the Bioregenerative Planetary Life Support Systems Test Complex (BIO-Plex) is currently being built at the NASA Johnson Space Center. The BIO-Plex facility will allow for closed chamber Earth-based tests. Various prepackaged food systems are being considered for the first 120-day BIO-Plex test. These food systems will be based on the Shuttle Training Menu and the International Space Station (ISS) Assembly Complete food systems. This paper evaluates several prepackaged food system options for the surface portion of an early Mars mission, based on plans for the first BIO-Plex test. The five systems considered are listed in Table 1. The food system options are assessed using equivalent system mass (ESM), which evaluates each option based upon the mass, volume, power, cooling and crewtime requirements.

Research into increased capacity solid amine sorbents has found a candidate (SA9T) that will provide enough increase in cyclic carbon dioxide removal capacity to produce a fully redundant Regenerative Carbon Dioxide Removal System (RCRS). This system will eliminate the need for large quantities of backup LiOH, thus gaining critical storage space on board the shuttle orbiter. This new sorbent has shown an ability to package two fully redundant (four) sorbent beds together with their respective valves, fans and plumbing to create two operationally independent systems. The increase in CO2 removal capacity of the new sorbent will allow these two systems to fit within the envelope presently used by the RCRS. This paper reports on the sub-scale amine testing performed in support of the development effort. In addition, this paper will provide a preliminary design schematic of a fully redundant RCRS.

This paper provides an overview of the IMMWPS Integrated Ground Test Program, completed at the NASA Johnson Space Center (JSC) during October and November 2001. The JSC Crew and Thermal Systems Division (CTSD) has developed the IMMWPS orbital flight experiment to test the feasibility of a microbe-based water purifier for use in zero-gravity conditions. The IMMWPS design utilizes a Microbial Processor Assembly (MPA) inoculated with facultative anaerobes to convert organic contaminants in wastewater to carbon dioxide and biomass. The primary purpose of the ground test program was to verify functional operations and procedures. A secondary objective was to provide initial ground data for later comparison to on-orbit performance. This paper provides a description of the overall test program, including the test article hardware and the test sequence performed to simulate the anticipated space flight test program. In addition, a summary of significant results from the testing is provided.

The quantitative analysis of the food system for long-term space missions is a crucial factor for the comparison of different food plans and for the evaluation of the food system as part of the overall mission. Such analysis should include important factors such as nutrition, palatability, diet cycle length, and psychological issues related to food. This paper will give the details of a mathematical model that was developed during the first author's participation as a Summer Faculty Fellow at Johnson Space Center. The model includes nutrition, palatability, diet cycle length, and psychological issues as important components. The model is compatible with the Equivalent System Mass (ESM) metric previously developed as the Advance Life Support (ALS) Research and Technology Metric.

The International Space Station Waste Collector Subsystem Risk Mitigation Experiment (ISS WCS RME) was flown as the primary (Shuttle) WCS on Space Shuttle flight STS-104 (ISS-7A) in July 2001, to validate new design enhancements. In general, the WCS is utilized for collecting, storing, and compacting fecal & associated personal hygiene waste, in a zero gravity environment. In addition, the WCS collects and transfers urine to the Shuttle waste storage tank. All functions are executed while controlling odors and providing crew comfort. The ISS WCS previously flew on three Shuttle flights as the Extended Duration Orbiter (EDO) WCS, as it was originally designed to support extended duration Space Shuttle flights up to 30 days in length. Soon after its third flight, the Space Shuttle Program decided to no longer require 30 day extended mission duration capability and provided the EDO WCS to the ISS Program.

Two crops of lettuce (Lactuca sativa cv. Waldmann's Green) were grown in the Regenerative Life Support Systems (RLSS) Test Bed at NASA's Johnson Space Center. The RLSS Test Bed is an atmospherically closed, controlled environment facility for the evaluation of regenerative life support systems using higher plants. The chamber encloses 10.6 m2 of growth area under cool-white fluorescent lamps. Lettuce was double seeded in 480 pots, each containing about 250 cm3 of calcined-clay substrate. Each pot was irrigated with half-strength Hoagland's nutrient solution at an average total applied amount of 2.5 and 1.8 liters pot-1, respectively, over each of the two 30-day crop tests. Average environmental and cultural conditions during both tests were 23°C air temperature, 72% relative humidity, 1000 ppm carbon dioxide (CO2), 16h light/8h dark photoperiod, and 356 μmol m-2s-1 photosynthetic photon flux.

During extravehicular activities (EVAs) it is crucial to keep the astronaut comfortable. Currently, a sublimator rejects to space both the astronaut's metabolic heat and that produced by the Portable Life Support System. In doing so, it consumes up to 3.6 kg (8 lbm) of water; the single largest expendable during an eight-hour EVA. While acceptable for low earth orbit, resupply for moon and interplanetary missions will be too costly. Fortunately, the amount of water consumed can be greatly reduced if most of the heat load is radiated to space. However, the radiator must reject heat at the same rate that it is generated to prevent heat stroke or frostbite. Herein, we report on a freezable radiator and heat exchanger to proportionally control the heat rejection rate.

Phase II of the Lunar-Mars Life Support Test Project was conducted in June and July of 1996 at the NASA Johnson Space Center. The primary objective for Phase II was to develop and test an integrated human life support system capable of sustaining a crew of four for 30 days in a closed chamber. The crew was continuously present inside a chamber throughout the 30-day test. The objective of this paper is to describe crew interactions and human factors for the test. Crew preparations for the test included training and familiarization of chamber systems and accommodations, and medical and psychological evaluations. During the test, crew members provided metabolic loads for the life support systems, performed maintenance on chamber systems, and evaluated human factors inside the chamber. Overall, the four crew members found the chamber to be comfortable for the 30-day test.

The Flash Evaporator Subsystem (FES) provides active cooling for the Shuttle Orbiter vehicle during the ascent and re-entry phases of the flight and provides supplemental cooling to the radiators while on-orbit. This paper describes the design and operation of the FES and summarizes the operational flight experience to date. As the fleet of orbiters grows older, contamination and corrosion are two issues on which attention has focused. A discussion of these conditions and the subsequent design changes and operational workarounds will be summarized.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

The Advanced Integration Matrix (AIM) project at the Johnson Space Center (JSC) was chartered to study and solve systems-level integration issues for exploration missions. One of the first issues identified was an inability to conduct trade studies on control system architectures due to the absence of mature evaluation criteria. Such architectures are necessary to enable integration of regenerative life support systems. A team was formed to address issues concerning software and hardware architectures and system controls.. The team has investigated what is required to integrate controls for the types of non-linear dynamic systems encountered in advanced life support. To this end, a water processing bioreactor testbed is being developed which will enable prototyping and testing of integration strategies and technologies.

Meeting radiation protection requirements during EVA is predominantly an operational issue with some potential considerations for temporary shelter. The issue of spacesuit shielding is mainly guided by the potential of accidental exposure when operational and temporary shelter considerations fail to maintain exposures within operational limits. In this case, very high exposure levels are possible which could result in observable health effects and even be life threatening. Under these assumptions, potential spacesuit radiation exposures have been studied using known historical solar particle events to gain insight on the usefulness of modification of spacesuit design in which the control of skin exposure is a critical design issue and reduction of blood forming organ exposure is desirable.

An ADvanced Automotive Manikin (ADAM) developed at the National Renewable Energy Laboratory (NREL) is used to evaluate NASA’s liquid cooling garments (LCGs) used in advanced spacesuits. The manikin has 120 separate heated/sweating zones and is controlled by a finite-element physiological model of the human thermo-regulatory system. Previous testing showed the thermal sensation and comfort followed expected trends as the LCG inlet fluid temperature was changed. The Phase II test data demonstrates the repeatability of ADAM by retesting the baseline LCG. Skin and core temperature predictions using ADAM in an LCG/arctic suit combination are compared to NASA physiological data to validate the manikin/model. An additional Orlan LCG configuration is assessed using the manikin and compared to the baseline LCG.

The challenges of missions to the Moon and Mars presents NASA with the need for more advanced life support systems, including better technologies for CO2 removal in spacecraft atmospheres and extravehicular mobility units (EMU). Amine-coated single wall carbon nanotubes (SWCNT) have been proposed as a potential solution because of their high surface area and thermal conductivity. Initial research demonstrated the need for functionalization of SWCNT to obtain optimal adherence of the amine to the SWCNT support phase [1]. Recent efforts focus on the development of new methods to chemically bond amines to SWCNT. Synthesis and characterization methods for these materials are discussed and some preliminary materials characterization data are presented. The CO2 adsorption capacity for several versions of SWCNT supported amine-based CO2 scrubber materials is also determined.